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polyfect transcription reagent  (Qiagen)


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    Structured Review

    Qiagen polyfect transcription reagent
    Polyfect Transcription Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 2437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyfect+transcription+reagent/PolyFect+Transfection+Reagent/pmc02794739-71-54-57
    Average 97 stars, based on 2437 article reviews
    polyfect transcription reagent - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Transfection:

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Luciferase Assay—Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBGvector to normalize the amount ofDNAper condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer’s instructions. ..

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Luciferase Assay Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..

    Luciferase:

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Luciferase Assay—Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBGvector to normalize the amount ofDNAper condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer’s instructions. ..

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Luciferase Assay Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..

    Construct:

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Luciferase Assay—Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBGvector to normalize the amount ofDNAper condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer’s instructions. ..

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Luciferase Assay Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..

    Plasmid Preparation:

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..

    Article Title: Williams-Beuren Syndrome-associated Transcription Factor TFII-I Regulates Osteogenic Marker Genes
    Article Snippet: .. Luciferase Assay Cos7 cells were transiently transfected with a combination of 600 ng of luciferase reporter construct mOG1.3 containing 1.3 kb of osteocalcin promoter; 0.35 ng of Renilla pRL-TK; and increasing amounts (250, 500, and 1000 ng) of wild type TFII-I (pEBG-TFII-I), FLAG-Runx2, HA-pRb, or pEBG vector to normalize the amount of DNA per condition, using PolyFect transcription reagent (Qiagen) according to the manufacturer's instructions. ..



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    97
    Qiagen polyfect transcription reagent
    Polyfect Transcription Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyfect+transcription+reagent/PolyFect+Transfection+Reagent/pmc02794739-71-54-57
    Average 97 stars, based on 1 article reviews
    polyfect transcription reagent - by Bioz Stars, 2026-10
    97/100 stars
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